Tissue Culturing

The technique of cleaning plants of disease, then multiplying in culture means a faster release of new cultivars (this is already achieved with potatoes) and healthier uniform plants with more uniform fruits or tubers.

 

 

New cultivars

and best lines of existing cultivars

             
1. INTRODUCTION

Tubers or Tissue culture plants.

       6. GREENHOUSE MULTIPLICATION

Stage 0 plantlets placed in Mistbed for 7 days, then planted in irrigated troughs of soil mix to grow and form tubers. Clones are virus tested. Tubers harvested, cool stored for field multiplication.
2. HEAT TREATMENT

Plants in pots at 36oC for 6-8 weeks.

      
3. TISSUE CULTURE

Meristem tips of side buds.

Under sterile conditions.

Subculture.

       7. FIELD MULTIPLICATION

Stage 1 at 360m; Stage 2-4 near Methven. 100 hectares under ALMAC control. % testing for virus.
4. PATHOGEN TESTING

Tissue culture plantlets and also also adult plants. ELISA testing for PVX, Y, S, M, A and PLRV. Indicator plants, Electron Microscope. Fungi and bacteria tests

        

5. TISSUE CULTURE MULTIPLICATION

Plantlet multiplication in culture.

INVITRO STORAGE

Plantlets stored on restricting media at low temperature.

 8. FIELD MULTIPLICATION BY FARMERS

Basic 1st and 2nd generation seed. Tableware. NZ SPCA inspection.
             
 

Pathogen tested potatoes

  
             
 

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